Journal: Life science alliance

Article Title: Kinesins regulate the heterogeneity in centrosome clustering after whole-genome duplication.

doi: 10.26508/lsa.202402670

Figure Lengend Snippet: Figure 3. Extra spindle poles increase KIF11–microtubule association. (A) Generation of stable tetraploids. Whole-genome duplication (WGD) was induced in HeLa cells as described in Fig 1A. Clones with a near-tetraploid DNA content and the normal number of centrosomes were isolated by limiting dilution (HeLa-T). (B) Freshly generated tetraploids and stable HeLa-T contain tetraploid DNA. The DNA contents of the two types of tetraploids (fixed immediately after WGD or after single-colony isolation) and normal HeLa cells were analyzed with flow cytometry. (C) Tetraploids contain higher levels of KIF11 and tubulin. The indicated cells were blocked in mitosis with nocodazole for 18 h followed by shake-off. Lysates from 2 × 104

Article Snippet: The following antibodies were obtained from the indicated sources: alpha-tubulin (EP1332Y) (ab52866; Abcam), Alexa Fluor 488–conjugated alpha-tubulin (11H10) (5063S; Cell Signaling Technology), beta-actin (AC-74) (A5316; Sigma-Aldrich), centrin1 (20H5) (04-1624; Sigma-Aldrich), phosphorylated histone H3Ser10 (sc-8656R; Santa Cruz Biotechnology), KIFC1 (12313S; Cell Signaling Technology), KIFC3 (D-9) (sc-365494; Santa Cruz Biotechnology), KIF11 (BD611186; BD Biosciences), KIF15 (36-1) (sc1009489; Santa Cruz Biotechnology), pericentrin (ab220784; Abcam), and SPC24 (A16601; ABclonal Technology).

Techniques: Clone Assay, Isolation, Generated, Cytometry

Journal: Nature immunology

Article Title: The receptor DNGR-1 signals for phagosomal rupture to promote cross-presentation of dead cell-associated antigens

doi: 10.1038/s41590-020-00824-x

Figure Lengend Snippet: Abbreviations: FC (flow cytometry), CI (cell isolation)

Article Snippet: Target Conjugate Clone Stock (mg/ml) Application Company CD4 PE RM4-5 0.2 CI 1:200 BD Biosciences 553049 CD8a BV785 53-6.7 0.2 FC: 1:200 Biolegend 100750 CD11b PE M1/70 0.2 CI: 1:200 BD Biosciences 553311 CD11c PE HL3 0.2 CI: 1:200 BD Biosciences 553802 CD16/32 PE 2.4G2 0.2 CI: 1:200 BD Biosciences 553145 CD40 PE 3/23 0.2 FC: 1:200 BD Biosciences 553791 CD45.1 AF647 A20 0.5 FC: 1:200 Biolegend 110720 CD45.2 BV510 104 0.2 FC: 1:200 Biolegend 109838 CD44 FITC 1M7 0.5 FC: 1:200 BD Pharmingen 553133 CD45R (B220) PE RA3-6B2 0.2 CI: 1:200 BD Biosciences 553090 CD62L PE-Cy7 MEL-14 0.2 FC 1:200 Biolegend 104418 TCRβ BV421 104 0.2 FC 1:200 Biolegend 109230 CD107b (LAMP-2) FITC M3/84 0.5 FC 1:200 Biolegend 108504 CD107b (LAMP-2) AF647 M3/84 0.5 FC 1:200 BD Biosciences 564843 DNGR-1 PE 1F6 0.2 FC: 1:50 Biolegend custom DNGR-1 APC 1F6 0.2 FC 1:50 Biolegend custom H-2K b (MHC I) APC AF6-88.5 0.2 FC 1:200 Biolegend 116518 H-2K d (MHC I) FITC SF1-1.1 0.2 FC 1:200 BD Pharmingen 553565 I-A/I-E (MHC II) BV711 M5/11 4.15.2 0.2 FC 1:200 BD Biosciences 563414 Kb/OVA257 (SIINFEKL) tetramer PE - 2 FC 1:400 Baylor College of Medicine Pro5® MHC pentamer H-2K b SIINFEKL R-PE - - FC 1:100 ProImmune Interferon-γ biotin XMG1.2 0.5 ELISA 1:1,000 BD Biosciences 554410 Interferon-γ PE XMG1.2 0.2 FC 1:100 Biolegend 505808 Ly6c/Ly6g (Gr-1) PE RB-8C5 0.2 CI 1:200 Biolegend 108408 XCR-1 PE ZET 0.2 CI 1:50 Biolegend 148204 alpha-Tubulin HRP 11H10 n/a WB: 1000 Cell Signaling 9099S Open in a separate window Abbreviations: FC (flow cytometry), CI (cell isolation)

Techniques: Flow Cytometry, Isolation, Enzyme-linked Immunosorbent Assay